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Image Search Results
Journal: bioRxiv
Article Title: Gb3 trisaccharide-bearing exosomes as a novel neutralizer for Shiga toxin type 1
doi: 10.1101/2024.10.26.620411
Figure Lengend Snippet: Flow cytometry analysis of (A) CHO-Lec2 cells transfected with vector encoding human α1,4-galactosyltransferase with p.Q211E substitution (A4G) or untransfected control (NAT) and (B) 4µm aldehyde/sulfate latex beads captured CHO-Lec2 NAT-derived exosomes treated with Gb3-FSL conjugate. The binding of the anti-P1 (clone 650) antibody and Stx1B-AF488 (coincubated with exo-Gb3-FSL or not) was examined ( A ). Aldehyde/sulfate latex beads with captured exosomes treated or not with Gb3-FSL conjugate were evaluated using anti-CD81 (clone 2F2), anti-P1 (clone 650) and Stx1B-AF488 ( B ).
Article Snippet: The exosomes-Gb3-FSL constructs were bound to aldehyde/latex beads, 4% w/v, 4 μm (Thermo Fisher Scientific, Inc., Waltham, MA, USA), and blocked with 100 mM glycine solution (Sigma-Aldrich, St. Louis, MO) and incubated with anti-P1 (clone 650), anti-CD81 (clone 2F2) antibodies as well as with
Techniques: Flow Cytometry, Transfection, Plasmid Preparation, Control, Derivative Assay, Binding Assay